| 產(chǎn)品編號 | bsm-60093M |
| 英文名稱 | Mono-Methyl-Histone H3 (Lys4) Recombinant Mouse mAb |
| 中文名稱 | |
| 產(chǎn)品類型 | 甲基化抗體 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號 | G4G10 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000,IHC-P=1:20-100,IHC-F=1:20-100,IF=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG2b/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白翻譯后修飾（PTMs）是表觀遺傳學(xué)調(diào)控染色質(zhì)結(jié)構(gòu)的關(guān)鍵機(jī)制，被稱為“組蛋白密碼”。組蛋白上的翻譯后修飾包括乙酰化，甲基化，磷酸化和近年發(fā)現(xiàn)的一些新型酰化修飾。這些組蛋白修飾直接影響染色質(zhì)和轉(zhuǎn)錄因子或其他表觀調(diào)控子的結(jié)合，改變基因組的穩(wěn)定性和基因轉(zhuǎn)錄等。組蛋白甲基化通常發(fā)生在核心組蛋白的賴氨酸和精氨酸殘基。組蛋白甲基化既可以促進(jìn)也可以抑制基因轉(zhuǎn)錄，這取決于組蛋白甲基化是發(fā)生在賴氨酸還是發(fā)生在精氨酸上以及甲基化基團(tuán)的個數(shù)（賴氨酸可以發(fā)生一、二、三甲基化，精氨酸可發(fā)生單、對稱與非對稱二甲化）。組蛋白賴氨酸甲基化通常發(fā)生在組蛋白H3第4，9，27，36，79和組蛋白H4的第20位賴氨酸上；精氨酸甲基化通常發(fā)生在組蛋白H3的第2，8，17，26和組蛋白H4第3位精氨酸上。蛋白甲基化酶（HMT）和組蛋白去甲基化酶（HDM）是甲基化修飾主要的調(diào)控因子。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HepG2, Neuro-2a, BRL, EL4.IL-2
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Tissue: Rat testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60093M
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60093M
Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60093M
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