| 產品編號 | bsm-60068M |
| 英文名稱 | Acetyl-Histone H4 (Lys12) Mouse mAb |
| 中文名稱 | |
| 產品類型 | 乙?；贵w |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | A3H5 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-1:1000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG1/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | 組蛋白翻譯后修飾（PTMs）是表觀遺傳學調控染色質結構的關鍵機制，被稱為“組蛋白密碼”。組蛋白上的PTMs包括乙?；?、甲基化、磷酸化和新的酰化直接影響染色質對轉錄因子的可及性和其他表觀遺傳調控，改變基因組的穩定性，基因轉錄等。組蛋白乙酰化主要發生在核心組蛋白氨基末端的多個賴氨酸殘基上，以響應各種刺激，在基因表達調控、DNA損傷修復、染色質動力學等方面發揮重要作用。大多數情況下，組蛋白H2A主要在Lys5、9、15和36位處發生乙酰化修飾。 H2B主要在Lys5、12、15、16和20位處發生乙酰化修飾。組蛋白H3主要在Lys4、9、14、18、23、27、56和79位處發生乙酰化修飾。組蛋白H4主要在Lys5、8、12、16和20位處發生乙?；揎?。組蛋白乙酰轉移酶（HATs）和組蛋白去乙?；福℉DACs）是主要的調節因子。 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa+BSA (+), HeLa+BSA (-)
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Rat stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60068M
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60068M
Tissue: Human colon cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60068M
Cell line: (A) HeLa, (B) HeLa+Trichostatin A (500ng/ml, 4hr)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:300
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60068M
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
