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PTP1B Recombinant Rabbit mAb (bsm-60701R)  
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產品編號 bsm-60701R
英文名稱 PTP1B Recombinant Rabbit mAb
中文名稱 蛋白酪氨酸磷酸酶-1B重組兔單抗
別    名 PTN1_HUMAN; Tyrosine-protein phosphatase non-receptor type 1; EC:3.1.3.48; PTPN1; Protein-tyrosine phosphatase 1B(PTP-1B);  
研究領域 腫瘤  免疫學  信號轉導  腫瘤細胞生物標志物  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 R1C7
交叉反應 Human
產品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 50 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PTP1B 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]


SWISS:
P18031

Gene ID:
5770

Database links:

Entrez Gene: 5770 Human

SwissProt: P18031 Human




產品圖片
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: MCF7, 2: HeLa, 3: HCT-116, 4: HepG2 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 50 kDa Observed MW: 50 kDa
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