| 產品編號 | bsm-34148M |
| 英文名稱 | CHGA Mouse mAb |
| 中文名稱 | 嗜鉻粒素A單克隆抗體 |
| 別 名 | Chromogranin-A; Chromogranin A; beta Granin; CGA; CHG A; Chromogranin A parathyroid secretory protein 1; Chromogranin A precursor; ChromograninA; Pancreastatin; Parastatin; Parathyroid secretory protein 1; Pituitary secretory protein I; SP I; SP1; SPI; Va |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 13H7 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | IHC-P=1:200-400,IHC-F=1:200-400,IF=1:200-400
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHGA |
| 亞 型 | IgG1, Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | PBS, pH7.2, 0.02% Porcolin 300, containing stabilizing protein. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008]. Function: Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. Subunit: Interacts with SCG3. Subcellular Location: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. Post-translational modifications: Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Similarity: Belongs to the chromogranin/secretogranin proteinfamily. SWISS: P10645 Gene ID: 1113 Database links: Entrez Gene: 1113 Human Entrez Gene: 12652 Mouse Omim: 118910 Human SwissProt: P10645 Human SwissProt: P26339 Mouse Unigene: 150793 Human 嗜鉻粒素A蛋白存在與很多組織中：胃腸道內分泌細胞和類癌、甲狀旁腺、垂體前葉、腎上腺髓質、神經節、副神經節、胰島、甲狀腺C細胞等組織和其來源的腫瘤都表達該成分.嗜鉻素A（CgA）屬于可溶性的酸性蛋白，主要存在于神經內分泌細胞的分泌顆粒中，也存在于含有分泌顆粒的內分泌細胞和神經內分泌細胞來源的腫瘤細胞中。檢測循環中的免疫反應CgA對神經內分泌瘤具有診斷意義。另外，CgA的免疫組織化學檢測對鑒定來源于神經內分泌的腫瘤起到輔助作用。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CHGA) Monoclonal Antibody, Unconjugated (bsm-34148M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CHGA) Monoclonal Antibody, Unconjugated (bsm-34148M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CHGA) Monoclonal Antibody, Unconjugated (bsm-34148M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
