| 產品編號 | bs-41408R |
| 英文名稱 | SARS-CoV-2 N protein Rabbit pAb |
| 中文名稱 | SARS冠狀病毒2核衣殼蛋白抗體 |
| 別 名 | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Prot |
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Specific References (4) | bs-41408R has been referenced in 4 publications.
[IF=6.107] Christina Wenzek. et al. CD47 restricts antiviral function of alveolar macrophages during influenza virus infection. ISCIENCE. 2022 Nov;:105540 ELISA ; Monkey.
[IF=5.125] Anissa Viveiros. et al. SARS-CoV-2 Infection Downregulates Myocardial ACE2 and Potentiates Cardiac Inflammation in Humans and Hamsters. AM J PHYSIOL-HEART C. 2022 Nov;(Articles in Press): IHC ; Hamster.
[IF=5.075] Yuting Wu. et al. Construction, Characterization, and Application of a Nonpathogenic Virus-like Model for SARS-CoV-2 Nucleocapsid Protein by Phage Display. TOXINS. 2022 Oct;14(10):683 WB ; Other.
[IF=4.501] Aleksandra Milewska. et al. HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV. J Virol. 2021 Jan;95(4):e01622-20 WB ; Human.
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| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: SARS-CoV-2) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | ≥1mg/ml |
| 免 疫 原 | Recombinant SARS-CoV-2 Nucleocapsid Protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 產品圖片 |
Sample:
Lane 1: SARS-CoV N protein(Cat# bs-49002P) at 50ng
Lane 2: SARS-CoV N protein(Cat# bs-49002P) at 25ng
Lane 3: SARS-CoV N protein(Cat# bs-49002P) at 15ng
Lane 4: SARS-CoV N protein(Cat# bs-49002P) at 10ng
Lane 5: SARS-CoV N protein(Cat# bs-49002P) at 5ng
Lane 6: SARS-CoV N protein(Cat# bs-49002P) at 2.5ng
Lane 7: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng
Lane 8: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng
Lane 9: SARS-CoV-2 N protein(Cat# bs-41408P) at 15ng
Lane 10: SARS-CoV-2 N protein(Cat# bs-41408P) at 10ng
Lane 11: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng
Lane 12:SARS-CoV-2 N protein(Cat# bs-41408P) at 2.5ng
Primary: Anti- SARS-CoV-2 N protein (bs-41408R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Sample:
Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng
Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng
Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 15ng
Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 10ng
Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng
Lane 6:SARS-CoV-2 N protein(Cat# bs-41408P) at 2.5ng
Primary: Anti- SARS-CoV-2 N protein (bs-41408R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
