| 產品編號 | bs-1537R-HRP |
| 英文名稱 | Rabbit Anti-NADPH/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的還原型輔酶Ⅱ/磷酸酰胺腺嘌呤二核苷酸抗體 |
| 別 名 | nicotinamide adenine dinucleotide phosphate; NADP; NADPH; TPNH; Triphosphopyridine nucleotide, reduced form; |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產品類型 | 小分子抗體 |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | |
| 產品應用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 0.833kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated NADPH |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: NDAPH （nicotinamide adenine dinucleotide phosphate) includes of Alpha-NADPH and Beta-NADPH. Beta-NADPH hase activity only. Beta-Nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt or triphosphopyridine nucleotide reduced tetrasodium salt, also known as Coenzyme Ⅱ. β-Nicotinamide adenine dinucleotide 2′-phosphate (NADP+) and β-Nicotinamide adenine dinucleotide 2′-phosphate, reduced (NADPH) comprise a coenzyme redox pair (NADP+:NADPH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. The NADP+/NADPH redox pair facilitates electron transfer in anabolic reactions such as lipid and cholesterol biosynthesis and fatty acyl chain elongation. The NADP+/NADPH redox pair is used in a variety of antioxidation mechanism where it protects agains reactive oxidation species accumulation. NADPH is generated in vivio by the pentose phosphate pathway (PPP). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. NADPH氧化酶廣泛存在于機體多個系統發揮重要的生理功能。在多種病理情況下，NADPH氧化酶可以在很多組織中催化合成大量活性氧，導致過度氧化應激，使組織細胞的功能下降。 NADPH氧化酶也是血管內皮細胞ROS產生的主要酶系,由其介導產生ROS在信息傳遞、基因轉錄、細胞生長和凋亡以及調節內皮依賴性的一氧化氮合酶脫偶聯等血管舒張中的作用,使其成為血管生物學中重要的部分,NADPH氧化酶一方面與血管舒張的機能異常有關，另一方面又影響到內皮細胞的結構、粘附分子的表達、滲透、成長及遷移等功能。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
