| 產品編號 | bs-0095R-HRP |
| 英文名稱 | Rabbit Anti-Calponin 1/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的鈣調節蛋白-1抗體 |
| 別 名 | Basic calponin; Calponin 1; Calponin-1; Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin1; Calponins basic; CNN 1; CNN1; Sm Calp; SMCC; CNN1_HUMAN; Calponin H1; Calponin1; Sm Calp; smooth muscle. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 心血管 信號轉導 干細胞 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Sheep, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 33kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calponin 1 C-terminus |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Calponin, a calmodulin, is a thin filament associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. Calponin is expressed in smooth muscle and tissues containing significant amounts of smooth muscle. Two isoforms of calponin exist whose molecular weights are 34kDa and 29kDa. Expression of the 29kDa form is primarily restricted to muscle of the urogenital tract. The expression of calponin has also been demonstrated in myoepithelial cells from benign and malignant breast lesions. Function: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity). Subunit: Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Tissue Specificity: Smooth muscle, and tissues containing significant amounts of smooth muscle. Similarity: Belongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain. Database links: Entrez Gene: 1264 Human Entrez Gene: 12797 Mouse Omim: 600806 Human SwissProt: P51911 Human SwissProt: Q08091 Mouse Unigene: 465929 Human Unigene: 4356 Mouse Unigene: 31788 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Calponin1是一種激動蛋白結合蛋白，是平滑肌特有的一種蛋白，可以與肌動蛋白、原肌球蛋白和鈣調素結合,具有抑制平滑肌細胞收縮的功能，主要用于平滑肌腫瘤和乳腺等組織中的肌上皮細胞分布的研究。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
