| 產品編號 | bs-11524R-HRP |
| 英文名稱 | Rabbit Anti-NHLH2/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的螺旋環螺旋蛋白2抗體 |
| 別 名 | HEN2; Nescient helix loop helix 2; NSCL; NSCL2; HEN2_HUMAN. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 細胞生物 神經生物學 信號轉導 轉錄調節因子 G蛋白偶聯受體 表觀遺傳學 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NHLH2 (1-50aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: The helix-loop-helix (HLH) structures are known motifs commonly found in membrane-active and DNA-binding proteins. The helix-loop-helix proteins HEN1 and HEN2 are DNA-binding proteins that may be involved in cell-type determination in the early nervous system. Studies of expression in normal tissues have demonstrated expression of NHLH1/NSCL-1 and NHLH2/NSCL-2, the genes encoding HEN1 and HEN2, in the developing central and peripheral nervous system, specifically in developing neurons. Function: NHLH2 are two basic domain helix-loop-helix (bHLH) proteins expressed predominantly in the developing nervous system and also in some cell lines derived from tumors with neural or neuroendocrine features such as neuroblastoma, PNET, and small cell lung cancer. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Subcellular Location: Nucleus Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. Database links: UniProtKB/Swiss-Prot: Q02577.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
