| 產(chǎn)品編號 | bs-3793R-BF647 |
| 英文名稱 | Rabbit Anti-CD75/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的唾液酸轉(zhuǎn)移酶1/α-2,6唾液酸轉(zhuǎn)移酶抗體 |
| 別 名 | 6 sialyltransferase; 6-sialyltransferase 1; 6-ST 1; Alpha 2; Alpha 2,6 ST 1; Alpha 2,6 ST; B cell antigen CD75; B-cell antigen CD75; Beta galactoside alpha 2,6 sialyltransferase 1; Beta-galactoside alpha-2; CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2; MGC48859; Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase); Sialyltransferase 1; SIAT1; SIAT1_HUMAN; ST6 beta galactosamide alpha 2,6 sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 免疫學 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)運蛋白 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=3ug/Test IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 47kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD75 (321-370aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. Function: Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Subcellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. Post-translational modifications: The soluble form derives from the membrane form by proteolytic processing. The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. Similarity: Belongs to the glycosyltransferase 29 family. Database links: Entrez Gene: 6480 Human Entrez Gene: 20440 Mouse Omim: 109675 Human SwissProt: P15907 Human SwissProt: Q64685 Mouse Unigene: 207459 Human Unigene: 149029 Mouse Unigene: 54567 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-CD75 Antibody, conjugated (bs-3793R-BF647) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-CD75 antibody (bs-3793R-BF647)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
