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Rabbit Anti-large S protein/AP Conjugated antibody (bs-0343R-AP)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-0343R-AP
英文名稱 Rabbit Anti-large S protein/AP Conjugated antibody
中文名稱 堿性磷酸酶(AP)標記的人乙肝病毒Large S蛋白抗體
別    名 HBSAG_HBVC5; preS1/preS2/S; Hepatitis B Virus Surface Antigen; Large envelope protein; L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen; Hepatitis B virus ad/Japan/S-179/1988.   
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 腫瘤  免疫學  細菌及病毒  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應
產品應用 WB=1:50-200 IHC-F=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 31/44kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from HBSAG_HBVC5
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
HBV surface proteins [Hepatitis B virus]. Major surface antigen from hepadnavirus; pfam00695.

Function:
The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

Subunit:
Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion.

Subcellular Location:
Virion membrane.

Post-translational modifications:
Isoform M is N-terminaly acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region.
Myristoylated.

Similarity:
Belongs to the orthohepadnavirus major surface antigen family.

Database links:
UniProtKB/Swiss-Prot: P03140.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

乙型肝肝炎病毒顆粒直徑42nm,由包膜與核衣殼組成,包膜含有碳水化合物、蛋白質和脂類。其中蛋白質為主要抗原成分,包括S抗原:pre-s1區和pre-s2區,這些抗原成分在病毒侵入細胞過程中有著至關重要的作用,為深入研究乙型肝肝炎病毒發病機理及變異有著至關重要的作用。
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