| 產品編號 | bs-0212R-HRP |
| 英文名稱 | Rabbit Anti-Hepatitis E Virus ORF3 /HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的戊型肝炎病毒抗體 |
| 別 名 | structural protein; immunogenic protein; ORF-3; ORF 3; Protein ORF3; pORF3; phosphoprotein; ORF3 [Hepatitis E virus]; ORF3_HEVHY. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 免疫學 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | |
| 產品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 13.5kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from HEV ORF3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: The Hepatitis E virus is the causative agent of Hepatitis E. Its taxonomic name is Orthohepevirus A. The viral genome a single-strand of positive-sense RNA that is approximately 7200 bases in length. It encodes 3 proteins (O1, O2, O3), two of which are polyproteins, that is, they are cleaved into fragments which carry out the actual functions of the virus. The O1 protein consists of 7 such fragments, namely Met (Methyltransferase), Y (Y-domain), Plp (Papain like protease), V (proline-rich variable region), X (X-domain, macro-domain), Hel (Helicase), and Rdrp (RNA dependent RNA polymerase). The Pvx domain is a fusion protein consisting of the Plp, V and X domains. The O3 protein is encoded by a single open reading frame (ORF3). The O2 protein encodes the capsid, which is composed of 3 domains, namely the shell domain (S) and two protruding domains (P1, P2). Numbers in the figure indicate positions in the RNA sequence. Function: May act as a viral regulatory protein involved in the modulation of mitogenic signaling pathways. May be involved in virion morphogenesis and viral pathogenesis. Expedites the processing and secretion of AMBP from the hepatocyte. Subunit: Homodimer. Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2 (via SH3 domain); binding does not activate the kinases. Interacts with host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts with host HPX/hemopexin. The phosphorylated form interacts with the unglycosylated capsid protein. Subcellular Location: Host cytoplasm, host cytoskeleton. Note=The N-terminal region seems to associate with the cytoskeleton probably via one of its hydrophobic regions. Post-translational modifications: Not glycosylated. Similarity: Belongs to the hepevirus ORF3 protein family. Database links:
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
