| 產(chǎn)品編號 | bs-1236R-HRP |
| 英文名稱 | Rabbit Anti-Involucrin/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的囊包蛋白/內(nèi)披蛋白抗體 |
| 別 名 | INVO_HUMAN; involucrin; ivl. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉(zhuǎn)導 細胞周期蛋白 細胞骨架 細胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, ) |
| 產(chǎn)品應用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 64kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Involucrin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]. Function: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Subunit: Directly or indirectly cross-linked to cornifelin (CNFN). Subcellular Location: Cytoplasm. Tissue Specificity: Keratinocytes of epidermis and other stratified squamous epithelia. Post-translational modifications: Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. Similarity: Belongs to the involucrin family. Database links: Entrez Gene: 3713 Human Entrez Gene: 16447 Mouse Omim: 147360 Human SwissProt: P07476 Human SwissProt: P48997 Mouse Unigene: 516439 Human Unigene: 207365 Mouse Unigene: 142591 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 套膜蛋白又稱內(nèi)披蛋白或囊包蛋白，與毛透明蛋白共同構(gòu)成角質(zhì)細胞包膜支架的蛋白質(zhì)。在角質(zhì)細胞分化時，首先出現(xiàn)在表皮棘層的上層細胞中的標志性蛋白質(zhì)，該蛋白在角質(zhì)細胞和其他復層扁平上皮合成的蛋白。最初存在于細胞質(zhì)中，然后通過轉(zhuǎn)谷氨酰胺酶的作用，轉(zhuǎn)變?yōu)榻宦?lián)的膜蛋白。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
