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Rabbit Anti-TOP1MT/AP Conjugated antibody (bs-1211R-AP)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-1211R-AP
英文名稱 Rabbit Anti-TOP1MT/AP Conjugated antibody
中文名稱 堿性磷酸酶(AP)標記的線粒體DNA拓普西異構酶Ⅰ抗體
別    名 Topoisomerase (DNA) I, mitochondrial; mitochondrial DNA topoisomerase I; DNA topoisomerase I, mitochondrial; TOP1mt; 2900052H09Rik; TOP1MT; TOP1M_HUMAN; DNA topoisomerase I, mitochondrial; mitochondrial; TOP1mt; Topoisomerase I mitochondrial.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 腫瘤  染色質和核信號  轉錄調節(jié)因子  激酶和磷酸酶  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse,  (predicted: Rat, )
產品應用 WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 66kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TOP1mt
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012].

Function:
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Subcellular Location:
Mitochondrion.

Tissue Specificity:
Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.

Similarity:
Belongs to the type IB topoisomerase family.

Database links:

Entrez Gene: 116447 Human

Omim: 606387 Human

SwissProt: Q969P6 Human

Unigene: 528574 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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