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Rabbit Anti-Proteasome 20S alpha + beta/AP Conjugated antibody (bs-9360R-AP)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-9360R-AP
英文名稱 Rabbit Anti-Proteasome 20S alpha + beta/AP Conjugated antibody
中文名稱 堿性磷酸酶(AP)標記的蛋白酶體PSMα+β抗體
別    名 Macropain; Multicatalytic endopeptidase complex subunit; Proteasome 20S alpha+beta; Prosome subunit; Proteasome subunit alpha type 1; Proteasome subunit alpha type 5; Proteasome subunit alpha type 7; Proteasome subunit; Proteasome subunit beta type 1; Proteasome subunit beta type 5; Proteasome subunit beta type 6; Proteasome subunit beta type 7; PSMA1; PSMA5; PSMA7; PSMB1; PSMB5; PSMB6; PSMB7; ZETA; PSA1_HUMAN.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  免疫學  細胞周期蛋白  細胞分化  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 (predicted: Human, Mouse, Rat, )
產品應用 WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 25-30kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from hu Proteasome 20S alpha + beta
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.
產品介紹 background:
The proteasome is widely recognised as the central enzyme of non lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP dependent pathway of protein degradation. The catalytic core of this unusually large (2000 kDa, 450?in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha subunits comprise the outer rings and the beta subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha7 beta7 beta7 alpha7) with a member of each sub family represented in each particle. Each subunit is located in a unique position within the alpha or beta rings. 20S Proteasomes degrade only unfolded proteins in an energy independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome.

Function:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009].

Subcellular Location:
Cytoplasmic and Nuclear.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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