| 產品編號 | bs-5205R-Bio |
| 英文名稱 | Rabbit Anti-Phospho-ATG4C(Ser398)/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的磷酸化自噬相關蛋白4C抗體 |
| 別 名 | APG4 autophagy 4 homolog C (S. cerevisiae); APG4 autophagy 4 homolog C; APG4 C; APG4-C; APG4C; ATG 4C; ATG4 autophagy related 4 homolog C (S. cerevisiae); Atg4c; ATG4C_HUMAN; AUT (S. cerevisiae) like 1, cysteine endopeptidase; AUT like 1, cysteine endopeptidase (S. cerevisiae); AUT like 1, cysteine endopeptidase; AUT like 3 cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; AUTL1; AUTL3; Autophagin 3; Autophagin-3; Autophagy related cysteine endopeptidase 3; Autophagy related protein 4 homolog C; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; Cysteine protease ATG4C; EC 3.4.22; FLJ14867; OTTHUMP00000010715. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 信號轉導 細胞凋亡 激酶和磷酸酶 線粒體 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, ) |
| 產品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 52kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human ATG4C around the phosphorylation site of Ser398 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Function: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Subcellular Location: Cytoplasm (Probable). Tissue Specificity: Highly expressed in skeletal muscle, heart, liver and testis. Similarity: Belongs to the peptidase C54 family. Database links: Entrez Gene: 84938 Human Entrez Gene: 242557 Mouse Omim: 611339 Human SwissProt: Q96DT6 Human SwissProt: Q811C2 Mouse Unigene: 7353 Human Unigene: 241663 Mouse Unigene: 23378 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
