| 產品編號 | bs-11851R-Bio |
| 英文名稱 | Rabbit Anti-HELT/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的轉錄因子HELT蛋白抗體 |
| 別 名 | Hairy and enhancer of split-related protein HELT; HCM1228; HELT; HELT_HUMAN; HES like ; HES/HEY like transcription factor; HES/HEY-like transcription factor; HESL; Heslike ; Hey like transcription factor; Hey like transcription factor (zebrafish); Hey like transcriptional repressor; Megane bHLH factor; Mgn. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 發育生物學 神經生物學 信號轉導 干細胞 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Cow, Horse, Rabbit, Zebrafish, Sheep, ) |
| 產品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 36kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HELT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: The Drosophila hairy and Enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The Notch signaling pathway is thought to maintain stem cells through transcriptional activation of HES/HEY family members to repress tissue-specific transcription factors. HESL (HES-like), also known as Mgn or HELT (HES/HEY-like transcription factor), is a 327 amino acid nuclear protein belonging to the HEY family. Containing a basic helix-loop-helix (bHLH) domain and an Orange domain, HESL self-associates and interacts with HES5 and HRT2. HESL is considered a transcriptional repressor that binds to the canonical E box sequence 5'-CACGCG-3'. HESL exists as two isoforms produced by alternative splicing events. Function: Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'. Subunit: Self-associates. Interacts with HES5 and HEY2 Subcellular Location: Nucleus. Similarity: Belongs to the HEY family. Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain. Database links: UniProtKB/Swiss-Prot: A6NFD8.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
