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Rabbit Anti-P2Y11/BF488 Conjugated antibody (bs-12071R-BF488)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價

產品編號 bs-12071R-BF488
英文名稱 Rabbit Anti-P2Y11/BF488 Conjugated antibody
中文名稱 BF488標記的G蛋白偶聯嘌呤受體p2y11抗體
別    名 P2RY11; P2Y purinoceptor 11; P2Y11; P2Y11 receptor; P2Y11_HUMAN; Purinergic receptor P2Y G protein coupled 11; Purinergic receptor P2Y11.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  神經生物學  通道蛋白  細胞膜受體  G蛋白偶聯受體  G蛋白信號  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,  (predicted: Pig, Cow, Horse, )
產品應用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 40kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human P2Y11
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
P2Y purinoceptor 11 (P2Y11) is a 374 amino acid protein belonging to the G-protein coupled receptor one family. P2Y11 is a multi-pass cell membrane protein that acts as a receptor for both ATP and ATD coupled to G proteins. Due to these interactions, P2Y11 is involved in phosphatidylinositol-calcium and adenylyl cyclase pathways. Induced by DMSO and retinoic acid, P2Y11 is highly expressed in spleen tissue. A putative trans-splicing event involving the gene that encodes P2Y11 and an upstream gene encoding PPAN has been found to result in a fusion protein, designated PPAN-P2RY11.

Function:
Receptor for ATP and ADP coupled to G-proteins that activate both phosphatidylinositol-calcium and adenylyl cyclase second messenger systems. Not activated by UTP or UDP.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Highest expression in liver and spleen.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

Database links:

Entrez Gene: 5032 Human

Omim: 602697 Human

SwissProt: Q96G91 Human

Unigene: 14468 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品圖片
Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Incubation: Anti-P2Y11 Polyclonal Antibody, conjugated (bs-12071R-BF488) 1:200, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
The Hela(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): BF488 conjugated Rabbit Anti-P2Y11 antibody (bs-12071R-BF488): 1 μg/10^6 cells; Isotype Control (orange): Rabbit-BF488 IgG (bs-0295P-BF488). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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