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Rabbit Anti-CBX4/BF647 Conjugated antibody (bs-17376R-BF647)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-17376R-BF647
英文名稱 Rabbit Anti-CBX4/BF647 Conjugated antibody
中文名稱 BF647標記的染色盒同源物4抗體
別    名 CBX 4; CBX4; Cbx4 chromobox homolog 4 (Drosophila Pc class); CBX4_HUMAN; Chromobox homolog 4 (Pc class homolog, Drosophila); Chromobox homolog 4; Chromobox protein homolog 4; E3 SUMO protein ligase CBX 4; E3 SUMO protein ligase CBX4; E3 SUMO-protein ligase CBX4; hPc 2; hPc2; NBP 16; NBP16; NS5ATP1 binding protein 16; Pc 2; Pc class 2 homolog; Pc class homolog; Pc class homolog Drosophila; PC2; Polycomb 2 homolog.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  結合蛋白  表觀遺傳學  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 (predicted: Human, Mouse, Rat, Dog, Pig, Horse, Sheep, )
產品應用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 61kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CBX4
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
Protamines are small, arginine-rich (basic), nuclear proteins that mediate normal sperm head condensation and DNA stabilization (1–3). Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine (3,4). The majority of DNA in human sperm is bound to protamines with only a small proportion of DNA bound to histones in a way similar to active chromatin (1,2). The retention of histone association with sperm DNA with respect to protamine association to sperm DNA can change within as little as 400 bp of DNA, suggesting that there is fine control over haploid DNA organization (1). Protamines eventually replace histones late in the haploid phase of spermatogenesis (1,2). The human protamine 1 gene maps to chromosome 16p13.13 and encodes a 51 amino acid protein (5). The human protamine 2 gene maps to chromosome 16p13.13 and encodes a 102 amino acid protein (6).

Function:
E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Subunit:
Interacts with histone H3-K9Me3. Interacts with CHTOP (By similarity). Component of a PRC1-like complex. Self-associates. Interacts with SUV39H1 and HIPK2. Interacts with CSNK2B.

Subcellular Location:
Nucleus.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494.

Similarity:
Contains 1 chromo domain.

Database links:

Entrez Gene: 8535 Human

Entrez Gene: 12418 Mouse

Entrez Gene: 501403 Rat

Omim: 603079 Human

SwissProt: O00257 Human

SwissProt: O55187 Mouse

Unigene: 268070 Mouse

Unigene: 471550 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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