| 產品編號 | bs-17894R-BF350 |
| 英文名稱 | Rabbit Anti-mtRNA polymerase/BF350 Conjugated antibody |
| 中文名稱 | BF350標記的線粒體RNA聚合酶抗體 |
| 別 名 | APOLMT; DNA-directed RNA polymerase; DNA-directed RNA polymerase mitochondrial; h-mtRPOL; mitochondrial; MTRNAP; MtRPOL; POLRMT; polymerase (RNA) mitochondrial (DNA directed); polymerase, RNA, mitochondrial; RPOM; RPOM_HUMAN. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 轉錄調節因子 新陳代謝 線粒體 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, (predicted: Mouse, Rat, ) |
| 產品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 134kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human mtRNA polymerase |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq, Jul 2008] Function: DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit: Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM. Subcellular Location: Mitochondrion. Similarity: Belongs to the phage and mitochondrial RNA polymerase family. Database links: Entrez Gene: 100723303 Guinea pig Entrez Gene: 5442 Human Omim: 601778 Human SwissProt: O00411 Human Unigene: 254113 Human Unigene: 24623 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
