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Mouse Anti-Gamma Tubulin/PE-Cy3 Conjugated antibody (bsm-33313M-PE-Cy3)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bsm-33313M-PE-Cy3
英文名稱 Mouse Anti-Gamma Tubulin/PE-Cy3 Conjugated antibody
中文名稱 PE-Cy3標(biāo)記的微管蛋白γ單克隆抗體
別    名 Centrosome Marker; Gamma 1 tubulin; gamma tubulin; gamma-tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; GCP 1; GCP-1; GCP1; MGC131994; TUBG; TUBG1; TUBG2; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Xgam; TBG1_HUMAN; TBG2_HUMAN; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1.  
規(guī)格價(jià)格 100ul/2980元 購買        大包裝/詢價(jià)
說 明 書 100ul  
產(chǎn)品類型 內(nèi)參抗體 
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  細(xì)胞骨架  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號(hào) 8D11
交叉反應(yīng) Mouse, Rat,  (predicted: Human, )
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 50kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Gamma Tubulin
亞    型 IgG
純化方法 affinity purified by Protein G
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.05M PB, pH 7.5.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Centrosome Marker
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.

Function:
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.

Subunit:
Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO.

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome.

Tissue Specificity:
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

Post-translational modifications:
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

Similarity:
Belongs to the tubulin family.

Database links:

Entrez Gene: 27175 Human

Entrez Gene: 7283 Human

Entrez Gene: 103733 Mouse

Entrez Gene: 103768 Mouse

Entrez Gene: 252921 Rat

Entrez Gene: 680991 Rat

Omim: 191135 Human

Omim: 605785 Human

SwissProt: P23258 Human

SwissProt: Q9NRH3 Human

SwissProt: P83887 Mouse

SwissProt: Q8VCK3 Mouse

SwissProt: P83888 Rat

Unigene: 279669 Human

Unigene: 708059 Human

Unigene: 142348 Mouse

Unigene: 479145 Mouse

Unigene: 154431 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

結(jié)構(gòu)蛋白(Structural Proteins)
gamma tubulin 微管蛋白是存于中心體的另一種微管蛋白, gamma微管蛋白對(duì)微管的形成具有重要作用。gamma微管蛋白通過與β-微管蛋白的相互作用幫助微管的成核反應(yīng)(nucleation)。即在微管的組裝中g(shù)amma微管蛋白先形成一個(gè)圓或形成鉤環(huán)結(jié)構(gòu), γ微管蛋白的這種結(jié)構(gòu)可指導(dǎo)微管蛋白二聚體結(jié)合上去并進(jìn)行微管的組裝。gamma微管蛋白在微管的組裝中起關(guān)鍵作用。
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